D Amino Acid Oxidase antibody (AA 20-347)

Catalog No. ABIN7600546

The Rabbit Polyclonal anti-D Amino Acid Oxidase antibody has been validated for WB, ELISA and FACS. It is suitable to detect D Amino Acid Oxidase in samples from Human and Rat.

Quick Overview for D Amino Acid Oxidase antibody (AA 20-347) (ABIN7600546)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This D Amino Acid Oxidase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-D Amino Acid Oxidase Antibody

Binding Specificity: AA 20-347

Purpose: Anti-DAO Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DAO Antibody Picoband® (ABIN7600546). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DAO recombinant protein (Position: H20-L347).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barker, R. F., Hopkinson, D. A. The genetic and biochemical properties of the D-amino acid oxidases in human tissues. Ann. Hum. Genet. 41: 27-42, 1977. 2. Chumakov, I., Blumenfeld, M., Guerassimenko, O., Cavarec, L., Palicio, M., Abderrahim, H., Bougueleret, L., Barry, C., Tanaka, H., La Rosa, P., Puech, A., Tahri, N., and 51 others. Genetic and physiological data implicating the new human gene G72 and the gene for D-amino acid oxidase in schizophrenia. Proc. Nat. Acad. Sci. 99: 13675-13680, 2002. Note: Erratum: Proc. Nat. Acad. Sci. 99: 17221 only, 2002. 3. de Belleroche, J., Morris, A. Reply to Millecamps et al.: elucidating the role of D amino oxidase in familial amyotrophic sclerosis. Proc. Nat. Acad. Sci. 107: E108, 2010. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: DAO

Background:

Synonyms: RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS,

Tissue Specificity: Ubiquitous.

Background: D-amino acid oxidase (DAAO, also OXDA, DAMOX) is an enzyme with the function on a molecular level to oxidize D-amino acids to the corresponding α-keto acids, producing ammonia and hydrogen peroxide. This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known, it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.

Molecular Weight: 36 kDa

Gene ID: 1610

UniProt: P14920