SF3A1 antibody (AA 20-556)

Catalog No. ABIN7600556

This anti-SF3A1 antibody is a Rabbit Polyclonal antibody detecting SF3A1 in WB, IHC, IF, ELISA and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for SF3A1 antibody (AA 20-556) (ABIN7600556)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-SF3A1 Antibody

Binding Specificity: AA 20-556

Purpose: Anti-SF3A1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SF3A1 Antibody Picoband® (ABIN7600556). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SF3A1 recombinant protein (Position: K20-Q556).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Isono, K., Abe, K., Tomaru, Y., Okazaki, Y., Hayashizaki, Y., Koseki, H. Molecular cloning, genetic mapping, and expression of the mouse Sf3b1 (SAP155) gene for the U2 snRNP component of spliceosome. Mammalian Genome 12: 192-198, 2001. 2. Kramer, A., Mulhauser, F., Wersig, C., Groning, K., Bilbe, G. Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae. RNA 1: 260-272, 1995. 3. Wang, C., Chua, K., Seghezzi, W., Lees, E., Gozani, O., Reed, R. Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis. Genes Dev. 12: 1409-1414, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Alternative Name: SF3A1

Background:

Synonyms: Serine protease HTRA3,3.4.21.-,High-temperature requirement factor A3,Pregnancy-related serine protease,HTRA3,PRSP,

Tissue Specificity: Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. In the endometrium, expressed in epithelial glands and the stroma. Also present in leukocytes. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney. .

Background: Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.

Molecular Weight: 120-130 kDa

Gene ID: 10291

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization