RUFY1 antibody (AA 20-650)

Catalog No. ABIN7600558

The Rabbit Polyclonal anti-RUFY1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect RUFY1 in samples from Human.

Quick Overview for RUFY1 antibody (AA 20-650) (ABIN7600558)

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUFY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RUFY1 Antibody

Binding Specificity: AA 20-650

Purpose: Anti-RUFY1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RUFY1 Antibody Picoband® (ABIN7600558). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RUFY1 recombinant protein (Position: L20-Q650).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Yang, J., Kim, O., Wu, J., Qiu, Y. Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1: a possible role of ETK in regulation of vesicle trafficking. J. Biol. Chem. 277: 30219-30226, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RUFY1

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Alternative Name: RUFY1

Background:

Synonyms: Protein NDRG3,N-myc downstream-regulated gene 3 protein,NDRG3,

Tissue Specificity: Ubiquitous. Highly expressed in brain. .

Background: RUN and FYVE domain-containing protein 1 is a protein that in humans is encoded by the RUFY1 gene. This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 80 kDa

Gene ID: 80230

UniProt: Q96T51