METAP2 antibody (AA 201-474)

Catalog No. ABIN7600568

This anti-METAP2 antibody is a Rabbit Polyclonal antibody detecting METAP2 in WB, ELISA and IHC. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for METAP2 antibody (AA 201-474) (ABIN7600568)

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-METAP2 Antibody

Binding Specificity: AA 201-474

Purpose: Anti-METAP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-METAP2 Antibody Picoband® (ABIN7600568). Tested in ELISA, IHC, WB, applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human METAP2 recombinant protein (Position: E201-R474).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry, 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Datta, B., Chakrabarti, D., Roy, A. L., Gupta, N. K. Roles of a 67- kDa polypeptide in reversal of protein synthesis inhibition in heme-deficient reticulocyte lysate. Proc. Nat. Acad. Sci. 85: 3324-3328, 1988. 2. Griffith, E. C., Su, Z., Niwayama, S., Ramsay, C. A., Chang, Y- H., Liu, J. O. Molecular recognition of angiogenesis inhibitors fumagillin and ovalicin by methionine aminopeptidase 2. Proc. Nat. Acad. Sci. 95: 15183-15188, 1998. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/21/2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for METAP2

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Alternative Name: METAP2

Background:

Synonyms: DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5, CDC46

Tissue Specificity: Ubiquitous.

Background: Methionine aminopeptidase 2 is an enzyme that in humans is encoded by the METAP2 gene. The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 67 kDa

Gene ID: 10988

UniProt: P50579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling