ACSS2 antibody (AA 201-651)

Catalog No. ABIN7600569

This Rabbit Polyclonal antibody specifically detects ACSS2 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACSS2 antibody (AA 201-651) (ABIN7600569)

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ACSS2 Antibody

Binding Specificity: AA 201-651

Purpose: Anti-ACSS2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACSS2 Antibody Picoband® (ABIN7600569). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACSS2 recombinant protein (Position: E201-A651).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fujino, T., Kondo, J., Ishikawa, M., Morikawa, K., Yamamoto, T. T. Acetyl-CoA synthetase 2, a mitochondrial matrix enzyme involved in the oxidation of acetate. J. Biol. Chem. 276: 11420-11426, 2001. 2. Luong, A., Hannah, V. C., Brown, M. S., Goldstein, J. L. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. J. Biol. Chem. 275: 26458-26466, 2000. 3. Mews, P., Donahue, G., Drake, A. M., Luczak, V., Abel, T., Berger, S. L. Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Nature 546: 381-386, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ACSS2

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Alternative Name: ACSS2

Background:

Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3

Tissue Specificity: Widely expressed.

Background: Acyl-coenzyme A synthetase short-chain family member 2 is an enzyme that in humans is encoded by the ACSS2 gene. This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.

Molecular Weight: 79 kDa

Gene ID: 55902