Spartin (SPART) (AA 205-631) antibody

Catalog No. ABIN7600578

This Rabbit Polyclonal antibody specifically detects in ELISA, WB and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for Spartin (SPART) (AA 205-631) antibody (ABIN7600578)

Target: Spartin (SPART)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 205-631

Purpose: Anti-SPART Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPART Antibody Picoband® (ABIN7600578). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPART recombinant protein (Position: H205-V631).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bakowska, J. C., Jenkins, R., Pendleton, J., Blackstone, C. The Troyer syndrome (SPG20) protein spartin interacts with Eps15. Biochem. Biophys. Res. Commun. 334: 1042-1048, 2005. 2. Bakowska, J. C., Wang, H., Xin, B., Sumner, C. J., Blackstone, C. Lack of spartin protein in Troyer syndrome: a loss-of-function disease mechanism? Arch. Neurol. 65: 520-524, 2008. 3. Butler, S., Helbig, K. L., Alcaraz, W., Seaver, L. H., Hsieh, D. T., Rohena, L. Three cases of Troyer syndrome in two families of Filipino descent. Am. J. Med. Genet. 170A: 1780-1785, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Spartin (SPART)

Alternative Name: SPART

Background:

Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29

Tissue Specificity: Ubiquitously expressed.

Background: Spartin is a protein that in humans is encoded by the SPG20 gene. This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome).

Molecular Weight: 78 kDa

Gene ID: 23111

UniProt: Q8N0X7