14-3-3 gamma antibody (AA 21-162)

Catalog No. ABIN7600596

This Rabbit Polyclonal antibody specifically detects 14-3-3 gamma in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 gamma antibody (AA 21-162) (ABIN7600596)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-14-3-3 gamma Antibody

Binding Specificity: AA 21-162

Purpose: Anti-14-3-3 gamma/YWHAG Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-14-3-3 gamma/YWHAG Antibody (ABIN7600596). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: D21-K162).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: YWHAG

Background:

Synonyms: Prostaglandin E synthase 3, Cytosolic prostaglandin E2 synthase, Cpges, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, PTGES3, P23, TEBP

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Molecular Weight: 28 kDa

Gene ID: 7532

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase