HCLS1 antibody (AA 213-473)

Catalog No. ABIN7600645

The Rabbit Polyclonal anti-HCLS1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect HCLS1 in samples from Human.

Quick Overview for HCLS1 antibody (AA 213-473) (ABIN7600645)

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCLS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-HCLS1 Antibody

Binding Specificity: AA 213-473

Purpose: Anti-HCLS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HCLS1 Antibody Picoband® (ABIN7600645). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HCLS1 recombinant protein (Position: N213-K473).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Egashira, M., Kitamura, D., Watanabe, T., Niikawa, N. The human HCLS1 gene maps to chromosome 3q13 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 72: 175-176, 1996. 2. Kitamura, D., Kaneko, H., Miyagoe, Y., Ariyasu, T., Watanabe, T. Isolation and characterization of a novel human gene expressed specifically in the cells of hematopoietic lineage. Nucleic Acids Res. 17: 9367-9379, 1989. 3. Scielzo, C., Ghia, P., Conti, A., Bachi, A., Guida, G., Geuna, M., Alessio, M., Caligaris-Cappio, F. HS1 protein is differentially expressed in chronic lymphocytic leukemia patient subsets with good or poor prognoses. J. Clin. Invest. 115: 1644-1650, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: HCLS1

Background:

Synonyms: Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD123, IL3RA, IL3R

Tissue Specificity: Brain.

Background: Hematopoietic lineage cell-specific protein is a protein that in humans is encoded by the HCLS1 gene. Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and protein kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction, positive regulation of protein phosphorylation, and regulation of transcription, DNA-templated. Located in cytosol, nucleus, and plasma membrane. Part of transcription regulator complex.

Molecular Weight: 75 kDa

Gene ID: 3059

UniProt: P14317

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location