Nucleolin antibody (AA 219-629)

Catalog No. ABIN7600662

This Rabbit Polyclonal antibody specifically detects Nucleolin in WB, IHC, IF, FACS, ELISA and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Nucleolin antibody (AA 219-629) (ABIN7600662)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 219-629

Purpose: Anti-Nucleolin/NCL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nucleolin/NCL Antibody Picoband® (ABIN7600662). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nucleolin/NCL recombinant protein (Position: K219-A629).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Harms, G., Kraft, R., Grelle, G., Volz, B., Dernedde, J., Tauber, R. Identification of nucleolin as a new L-selectin ligand. Biochem. J. 360: 531-538, 2001. 2. Hirano, K., Miki, Y., Hirai, Y., Sato, R., Itoh, T., Hayashi, A., Yamanaka, M., Eda, S., Beppu, M. A multifunctional shuttling protein nucleolin is a macrophage receptor for apoptotic cells. J. Biol. Chem. 280: 39284-39293, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background:

Synonyms: Nucleolin, Protein C23, NCL

Tissue Specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Background: Nucleolin is a protein that in humans is encoded by the NCL gene. It is mapped to 2q37.1. Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molecular Weight: 100-110 kDa

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization