NOTCH2 antibody (AA 2194-2471)

Catalog No. ABIN7600664

This anti-NOTCH2 antibody is a Rabbit Polyclonal antibody detecting NOTCH2 in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NOTCH2 antibody (AA 2194-2471) (ABIN7600664)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NOTCH2 Antibody

Binding Specificity: AA 2194-2471

Purpose: Anti-NOTCH2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOTCH2 Antibody Picoband® (ABIN7600664). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOTCH2 recombinant protein (Position: H2194-A2471).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Blaumueller, C. M., Qi, H., Zagouras, P., Artavanis-Tsakonas, S. Fixed cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell 90: 281-291, 1997. 2. Gao, X., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Gridley, T. Assignment of the murine Notch2 and Notch3 genes to chromosomes 3 and 17. Genomics 49: 160-161, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2

Background:

Synonyms: Neurogenic locus notch homolog protein 2, Notch 2, Hn2, Notch 2 extracellular truncation, N2ECD, Notch 2 intracellular domain, N2ICD, NOTCH2

Tissue Specificity: Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.

Background: Neurogenic locus notch homolog protein 2 also known as notch 2 is a protein that in humans is encoded by the NOTCH2 gene. It is mapped to 1p12. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 110 kDa, 300 kDa

Gene ID: 4853

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance