CD3D antibody (AA 22-171)

Catalog No. ABIN7600678

The Rabbit Polyclonal anti-CD3D antibody has been validated for WB, ELISA and FACS. It is suitable to detect CD3D in samples from Human.

Quick Overview for CD3D antibody (AA 22-171) (ABIN7600678)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CD3D Antibody

Binding Specificity: AA 22-171

Purpose: Anti-CD3D Antibody

Characteristics: Anti-CD3D Antibody (ABIN7600678). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD3D recombinant protein (Position: F22-K171). Human CD3D shares 66% and 70.7% amino acid (aa) sequence identity with mouse and rat CD3D, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.2-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background: T-cell surface glycoprotein CD3 delta chain is a protein that in humans is encoded by the CD3D gene. The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Molecular Weight: 19 kDa

Gene ID: 915

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events