CD3D antibody (AA 22-171)

Catalog No. ABIN7600678

The Rabbit Polyclonal anti-CD3D antibody has been validated for WB, ELISA and FACS. It is suitable to detect CD3D in samples from Human.

Quick Overview for CD3D antibody (AA 22-171) (ABIN7600678)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CD3D Antibody

Binding Specificity: AA 22-171

Purpose: Anti-CD3D Antibody Picoband®

Characteristics: Anti-CD3D Antibody Picoband® (ABIN7600678). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD3D recombinant protein (Position: F22-K171). Human CD3D shares 66% and 70.7% amino acid (aa) sequence identity with mouse and rat CD3D, respectively.

Application Details

Application Notes: Western blot, 0.2-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dadi, H. K., Simon, A. J., Roifman, C. M. Effect of CD3-delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency. New Eng. J. Med. 349: 1821-1828, 2003. Note: Erratum: New Eng. J. Med. 350: 1803 only, 2004. 2. de Saint Basile, G., Geissmann, F., Flori, E., Uring-Lambert, B., Soudais, C., Cavazzana-Calvo, M., Durandy, A., Jabado, N., Fischer, A., Le Diest, F. Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3. J. Clin. Invest. 114: 1512-1517, 2004. 3. Delgado, P., Fernandez, E., Dave, V., Kappes, D., Alarcon, B. CD3-delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection. Nature 406: 426-430, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background: T-cell surface glycoprotein CD3 delta chain is a protein that in humans is encoded by the CD3D gene. The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Molecular Weight: 19 kDa

Gene ID: 915

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events