MNT antibody (AA 222-582)

Catalog No. ABIN7600710

This Rabbit Polyclonal antibody specifically detects MNT in WB, ELISA, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for MNT antibody (AA 222-582) (ABIN7600710)

Target: MNT (MNT, MAX Dimerization Protein (MNT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MNT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MNT Antibody

Binding Specificity: AA 222-582

Purpose: Anti-MNT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MNT Antibody Picoband® (ABIN7600710). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MNT recombinant protein (Position: R222-A582).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hurlin, P. J., Queva, C., Eisenman, R. N. Mnt, a novel Max-interacting protein is coexpressed with Myc in proliferating cells and mediates repression at Myc binding sites. Genes Dev. 11: 44-58, 1997. 2. Lo Nigro, C., Venesio, T., Reymond, A., Meroni, G., Alberici, P., Cainarca, S., Enrico, F., Stack, M., Ledbetter, D. H., Liscia, D. S., Ballabio, A., Carrozzo, R. The human ROX gene: genomic structure and mutation analysis in human breast tumors. Genomics 49: 275-282, 1998. 3. Meroni, G., Reymond, A., Alcalay, M., Borsani, G., Tanigami, A., Tonlorenzi, R., Lo Nigro, C., Messali, S., Zollo, M., Ledbetter, D. H., Brent, R., Ballabio, A., Carrozzo, R. Rox, a novel bHLHZip protein expressed in quiescent cells that heterodimerizes with Max, binds a non-canonical E box and acts as a transcriptional repressor. EMBO J. 16: 2892-2906, 1997. Note: Erratum: EMBO J. 16: 6055 only, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MNT

Target: MNT (MNT, MAX Dimerization Protein (MNT))

Alternative Name: MNT

Background:

Synonyms: Max-binding protein MNT, Class D basic helix-loop-helix protein 3, bHLHd3, Myc antagonist MNT, Protein ROX, MNT, BHLHD3, ROX

Tissue Specificity: Expressed in some normal epithelial tissues and in some carcinoma cell lines.

Background: MNT (Max-binding protein MNT) is a Max-binding protein that is encoded by the MNT gene The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain.

Molecular Weight: 62 kDa

Gene ID: 4335

UniProt: Q99583

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation