L1CAM antibody (AA 223-1218)

Catalog No. ABIN7600711

The Rabbit Polyclonal anti-L1CAM antibody has been validated for WB, FACS and ELISA. It is suitable to detect L1CAM in samples from Human and Rat.

Quick Overview for L1CAM antibody (AA 223-1218) (ABIN7600711)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-L1CAM Antibody

Binding Specificity: AA 223-1218

Purpose: Anti-L1CAM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-L1CAM Antibody Picoband® (ABIN7600711). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human L1CAM recombinant protein (Position: E223-Q1218).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Basel-Vanagaite, L., Straussberg, R., Friez, M. J., Inbar, D., Korenreich, L., Shohat, M., Schwartz, C. E. Expanding the phenotypic spectrum of L1CAM-associated disease. Clin. Genet. 69: 414-419, 2006. 2. Du, Y.-Z., Dickerson, C., Aylsworth, A. S., Schwartz, C. E. A silent mutation, C924T (G308G), in the L1CAM gene results in X linked hydrocephalus (HSAS). J. Med. Genet. 35: 456-462, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: L1CAM

Background:

Synonyms: Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, CD171, L1CAM, CAML1, MIC5

Tissue Specificity: Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Background: L1, also known as L1CAM, is a transmembrane protein member of the L1 protein family, encoded by the L1CAM gene. The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

Molecular Weight: 220 kDa

Gene ID: 3897

UniProt: P32004

Pathways: Synaptic Membrane