C22orf28 antibody (AA 227-505)

Catalog No. ABIN7600722

This Rabbit Polyclonal antibody specifically detects C22orf28 in ELISA, WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for C22orf28 antibody (AA 227-505) (ABIN7600722)

Target: C22orf28 (Chromosome 22 Open Reading Frame 28 (C22orf28))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C22orf28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-C22orf28 Antibody

Binding Specificity: AA 227-505

Purpose: Anti-RTCB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RTCB Antibody Picoband® (ABIN7600722). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTCB recombinant protein (Position: H227-G505).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/14/2011. 2. Kanai, Y., Dohmae, N., Hirokawa, N. Kinesin transports RNA: isolation and characterization of an RNA-transporting granule. Neuron 43: 513-525, 2004. 3. Perez-Gonzalez, A., Pazo, A., Navajas, R., Ciordia, S., Rodriguez-Frandsen, A., Nieto, A. hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PLoS One 9: e90957, 2014. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for C22orf28

Target: C22orf28 (Chromosome 22 Open Reading Frame 28 (C22orf28))

Alternative Name: RTCB

Background:

Synonyms: Matrix metalloproteinase-16, MMP-16, 3.4.24.-, MMP-X2, Membrane-type matrix metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 matrix metalloproteinase, MT3-MMP, MT3MMP, MMP16, MMPX2,

Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Background: RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene. Enables RNA ligase (ATP) activity and vinculin binding activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytosol and nucleoplasm. Part of tRNA-splicing ligase complex.

Molecular Weight: 55 kDa

Gene ID: 51493

UniProt: Q9Y3I0