APOL1 antibody (AA 228-398)

Catalog No. ABIN7600725

This Rabbit Polyclonal antibody specifically detects APOL1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for APOL1 antibody (AA 228-398) (ABIN7600725)

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-APOL1 Antibody

Binding Specificity: AA 228-398

Purpose: Anti-Apolipoprotein L1/APOL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Apolipoprotein L1/APOL1 Antibody Picoband® (ABIN7600725). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Apolipoprotein L1/APOL1 recombinant protein (Position: M228-L398).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Page NM, Butlin DJ, Lomthaisong K, Lowry PJ (May 2001). "The human apolipoprotein L gene cluster: identification, classification, and sites of distribution". Genomics. 74 (1): 71-8. 2. Pérez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homblé F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E (July 2005). "Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes". Science. 309 (5733): 469-72. 3. Monajemi H, Fontijn RD, Pannekoek H, Horrevoets AJ (April 2002). "The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue". Genomics. 79 (4): 539-46.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for APOL1

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Alternative Name: APOL1

Background:

Synonyms: Apolipoprotein L1, Apolipoprotein L, Apo-L, ApoL, Apolipoprotein L-I, ApoL-I, APOL1, APOL

Tissue Specificity: Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.

Background: Apolipoprotein L1 is a protein that in humans is encoded by the APOL1 gene. It is mapped to 22q12.3. This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 8542

UniProt: O14791