FIP1L1 antibody (AA 23-558)

Catalog No. ABIN7600761

This Rabbit Polyclonal antibody specifically detects FIP1L1 in WB, IHC, IF, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FIP1L1 antibody (AA 23-558) (ABIN7600761)

Target: FIP1L1 (FIP1 Like 1 (FIP1L1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FIP1L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-FIP1L1 Antibody

Binding Specificity: AA 23-558

Purpose: Anti-FIP1L1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FIP1L1 Antibody Picoband® (ABIN7600761). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FIP1L1 recombinant protein (Position: E23-E558).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cools, J., DeAngelo, D. J., Gotlib, J., Stover, E. H., Legare, R. D., Cortes, J., Kutok, J., Clark, J., Galinsky, I., Griffin, J. D., Cross, N. C. P., Tefferi, A., and 14 others. A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome. New Eng. J. Med. 348: 1201-1214, 2003. 2. Griffin, J. H., Leung, J., Bruner, R. J., Caligiuri, M. A., Briesewitz, R. Discovery of a fusion kinase in EOL-1 cells and idiopathic hypereosinophilic syndrome. Proc. Nat. Acad. Sci. 100: 7830-7835, 2003. 3. Preker, P. J., Lingner, J., Minvielle-Sebastia, L., Keller, W. The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that ly interacts with poly(A) polymerase. Cell 81: 379-389, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FIP1L1

Target: FIP1L1 (FIP1 Like 1 (FIP1L1))

Alternative Name: FIP1L1

Background:

Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Background: Factor interacting with PAPOLA and CPSF1 (i.e, FIP1L1, also termed Pre-mRNA 3'-end-processing factor FIP1) is a protein that in humans is encoded by the FIP1L1 gene (also known as Rhe, FIP1, and hFip1). This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 72 kDa

Gene ID: 81608