STK26/MST4 antibody (AA 233-416)

Catalog No. ABIN7600780

The Rabbit Polyclonal anti-STK26/MST4 antibody has been validated for WB, ELISA and FACS. It is suitable to detect STK26/MST4 in samples from Human and Rat.

Quick Overview for STK26/MST4 antibody (AA 233-416) (ABIN7600780)

Target: STK26/MST4 (MST4) (Serine/threonine-Protein Kinase MST4 (MST4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK26/MST4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-STK26/MST4 Antibody

Binding Specificity: AA 233-416

Purpose: Anti-MST4/STK26 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MST4/STK26 Antibody Picoband® (ABIN7600780). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MST4/STK26 recombinant protein (Position: K233-P416).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. An, L., Nie, P., Chen, M., Tang, Y., Zhang, H., Guan, J., Cao, Z., Hou, C., Wang, W., Zhao, Y., Xu, H., Jiao, S., Zhou, Z. MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway. J. Exp. Med. 217: e20191817, 2020. Note: Electronic Article. 2. Dan, I., Ong, S.-E., Watanabe, N. M., Blagoev, B., Nielsen, M. M., Kajikawa, E., Kristiansen, T. Z., Mann, M., Pandey, A. Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties. J. Biol. Chem. 277: 5929-5939, 2002. 3. Qian, Z., Lin, C., Espinosa, R., LeBeau, M., Rosner, M. R. Cloning and characterization of MST4, a novel Ste20-like kinase. J. Biol. Chem. 276: 22439-22445, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for STK26/MST4

Target: STK26/MST4 (MST4) (Serine/threonine-Protein Kinase MST4 (MST4))

Alternative Name: STK26

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Molecular Weight: 52 kDa

Gene ID: 51765

UniProt: Q9P289