RORB antibody (AA 236-470)

Catalog No. ABIN7600788

This Rabbit Polyclonal antibody specifically detects RORB in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for RORB antibody (AA 236-470) (ABIN7600788)

Target: RORB (RAR-Related Orphan Receptor B (RORB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RORB Antibody

Binding Specificity: AA 236-470

Purpose: Anti-ROR beta/RORB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ROR beta/RORB Antibody Picoband® (ABIN7600788). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ROR beta/RORB recombinant protein (Position: E236-K470).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andre, E., Conquet, F., Steinmayr, M., Stratton, S. C., Porciatti, V., Becker-Andre, M. Disruption of retinoid-related orphan receptor beta changes circadian behavior, causes retinal degeneration and leads to vacillans phenotype in mice. EMBO J. 17: 3867-3877, 1998. 2. Baler, R., Coon, S., Klein, D. C. Orphan nuclear receptor RZR-beta: cyclic AMP regulates expression in the pineal gland. Biochem. Biophys. Res. Commun. 220: 975-978, 1996. 3. Carlberg, C., Hooft van Huijsduijnen, R., Staple, J. K., DeLamarter, J. F., Becker-Andre, M. RZRs, a new family of retinoid-related orphan receptors that function as both monomers and homodimers. Molec. Endocr. 8: 757-770, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RORB

Target: RORB (RAR-Related Orphan Receptor B (RORB))

Alternative Name: RORB

Background:

Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: RAR-related orphan receptor beta (ROR-beta), also known as NR1F2 (nuclear receptor subfamily 1, group F, member 2) is a nuclear receptor that in humans is encoded by the RORB gene. The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, and to help regulate the expression of some genes involved in circadian rhythm.

Molecular Weight: 53 kDa

Gene ID: 6096

UniProt: Q92753

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway