RALBP1 antibody (AA 239-506)

Catalog No. ABIN7600800

This Mouse Monoclonal antibody specifically detects RALBP1 in WB, IHC, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RALBP1 antibody (AA 239-506) (ABIN7600800)

Target: RALBP1 (RalA Binding Protein 1 (RALBP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RALBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2F9D3

Product Details anti-RALBP1 Antibody

Binding Specificity: AA 239-506

Purpose: Anti-RALBP1 Antibody Picoband® (monoclonal, 2F9D3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RALBP1 Antibody Picoband® (monoclonal, 2F9D3) (ABIN7600800). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RALBP1 recombinant protein (Position: K239-Q506).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: RALBP1 ralA binding protein 1". 2. Kashatus, D. F., Lim, K.-H., Brady, D. C., Pershing, N. L. K., Cox, A. D., Counter, C. M. RALA and RALBP1 regulate mitochondrial fission at mitosis. Nature Cell Biol. 13: 1108-1115, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RALBP1

Target: RALBP1 (RalA Binding Protein 1 (RALBP1))

Alternative Name: RALBP1

Background:

Synonyms: B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein, CD79b, CD79B, B29, IGB

Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Background: RalA-binding protein 1 is a protein that in humans is encoded by the RALBP1 gene. Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS, which in turn is activated by RAS. RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL. RALBP1 is also the dominant transporter of lipid peroxidation-derived glutathione conjugates and participates in several mitotic events, including inactivation of endocytosis and separation and polar movement of centrioles and appropriate distribution of mitochondria to daughter cells following mitosis.

Molecular Weight: 95 kDa

Gene ID: 10928

UniProt: Q15311