CCL21 antibody (AA 24-133)

Catalog No. ABIN7600807

The Rabbit Polyclonal anti-CCL21 antibody has been validated for WB, ELISA and FACS. It is suitable to detect CCL21 in samples from Mouse and Rat.

Quick Overview for CCL21 antibody (AA 24-133) (ABIN7600807)

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CCL21 Antibody

Binding Specificity: AA 24-133

Purpose: Anti-Ccl21 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Ccl21 Antibody Picoband® (ABIN7600807). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat Ccl21 recombinant protein (Position: S24-Q133).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/mL, Rat
1. Dijkstra, I. M., Hulshof, S., van der Valk, P., Boddeke, H. W. G. M., Biber, K. Cutting edge: activity of human adult microglia in response to CC chemokine ligand 21. J. Immun. 172: 2744-2747, 2004. 2. Harhausen, D., Prinz, V., Ziegler, G., Gertz, K., Endres, M., Lehrach, H., Gasque, P., Botto, M., Stahel, P. F., Dirnagl, U., Nietfeld, W., Trendelenburg, G. CD93/AA4.1: a novel regulator of inflammation in murine focal cerebral ischemia. J. Immun. 184: 6407-6417, 2010. 3. Hedrick, J. A., Zlotnik, A. Identification and characterization of a novel beta chemokine containing six conserved cysteines. J. Immun. 159: 1589-1593, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: Ccl21

Background:

Synonyms: Muellerian-inhibiting factor,Anti-Muellerian hormone,AMH,Muellerian-inhibiting substance,MIS,AMH,MIF,

Tissue Specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.

Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

Molecular Weight: 15 kDa

Gene ID: 298006

Pathways: Regulation of Actin Filament Polymerization