CD27 antibody (AA 24-187)

Catalog No. ABIN7600813

The Rabbit Polyclonal anti-CD27 antibody has been validated for FACS, ELISA and IHC. It is suitable to detect CD27 in samples from Mouse and Rat.

Quick Overview for CD27 antibody (AA 24-187) (ABIN7600813)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Product Details anti-CD27 Antibody

Binding Specificity: AA 24-187

Purpose: Anti-Cd27 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cd27 Antibody Picoband® (ABIN7600813). Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cd27 recombinant protein (Position: P24-F187).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: CD27 CD27 Molecule". 2. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF (Jun 1997). "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein". Proceedings of the National Academy of Sciences of the United States of America 94 (12): 6346-51. 3. Yamamoto H, Kishimoto T, Minamoto S (Nov 1998). "NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27". Journal of Immunology 161 (9): 4753-9.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: Cd27

Background:

Synonyms: Angiopoietin-related protein 4,Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ANGPTL4,ARP4, HFARP, PGAR,PP1158, PSEC0166, UNQ171/PRO197,

Tissue Specificity: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. .

Background: CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Molecular Weight: 45 kDa

Gene ID: 21940

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints