TSPYL5 antibody (AA 24-337)

Catalog No. ABIN7600825

The Rabbit Polyclonal anti-TSPYL5 antibody has been validated for WB, FACS and ELISA. It is suitable to detect TSPYL5 in samples from Human.

Quick Overview for TSPYL5 antibody (AA 24-337) (ABIN7600825)

Target: TSPYL5 (TSPY-Like 5 (TSPYL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSPYL5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-TSPYL5 Antibody

Binding Specificity: AA 24-337

Purpose: Anti-TSPYL5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TSPYL5 Antibody Picoband® (ABIN7600825). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TSPYL5 recombinant protein (Position: R24-Q337).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Epping, M. T., Meijer, L. A. T., Krijgsman, O., Bos, J. L., Pandolfi, P. P., Bernards, R. TSPYL5 suppresses p53 levels and function by physical interaction with USP7. Nature Cell Biol. 13: 102-108, 2011. 2. Jung, Y., Park, J., Bang, Y.-J., Kim, T.-Y. Gene silencing of TSPYL5 mediated by aberrant promoter methylation in gastric cancers. Lab. Invest. 88: 153-160, 2008. 3. Kim, E. J., Lee, S. Y., Kim, T. R., Choi, S. I., Cho, E. W., Kim, K. C., Kim, I. G. TSPYL5 is involved in cell growth and the resistance to radiation in A549 cells via the regulation of p21(WAF1/Cip1) and PTEN/AKT pathway. Biochem. Biophys. Res. Commun. 392: 448-453, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TSPYL5

Target: TSPYL5 (TSPY-Like 5 (TSPYL5))

Alternative Name: TSPYL5

Background:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: TSPY like 5 is a protein that in humans is encoded by the TSPYL5 gene. Predicted to enable chromatin binding activity and histone binding activity. Involved in several processes, including cellular response to gamma radiation, positive regulation of protein kinase B signaling, and positive regulation of protein ubiquitination. Predicted to be active in chromatin and nucleus.

Molecular Weight: 48 kDa

Gene ID: 85453