DLK1 antibody (AA 24-383)

Catalog No. ABIN7600828

The Rabbit Polyclonal anti-DLK1 antibody has been validated for WB and ELISA. It is suitable to detect DLK1 in samples from Human.

Quick Overview for DLK1 antibody (AA 24-383) (ABIN7600828)

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DLK1 Antibody

Binding Specificity: AA 24-383

Purpose: Anti-DLK-1/DLK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DLK-1/DLK1 Antibody Picoband® (ABIN7600828). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DLK-1/DLK1 recombinant protein (Position: A24-I383).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cleaton, M. A. M., Dent, C. L., Howard, M., Corish, J. A., Gutteridge, I., Sovio, U., Gaccioli, F., Takahashi, N., Bauer, S. R., Charnock-Jones, D. S., Powell, T. L., Smith, G. C. S., Ferguson-Smith, A. C., Charalambous, M. Fetus-derived DLK1 is required for maternal metabolic adaptations to pregnancy and is associated with fetal growth restriction. Nature Genet. 48: 1473-1480, 2016. 2. Cockett, N. E., Jackson, S. P., Shay, T. L., Farnir, F., Berghmans, S., Snowder, G. D., Nielsen, D. M., Georges, M. Polar overdominance at the ovine callipyge locus. Science 273: 236-238, 1996. 3. Dauber, A., Cunha-Silva, M., Macedo, D. B., Brito, V. N., Abreu, A. P., Roberts, S. A., Montenegro, L. R., Andrew, M., Kirby, A., Weirauch, M. T., Labilloy, G., Bessa, D. S., Carroll, R. S., Jacobs, D. C., Chappell, P. E., Mendonca, B. B., Haig, D., Kaiser, U. B., Latronico, A. C. Paternally inherited DLK1 deletion associated with familial central precocious puberty. J. Clin. Endocr. Metab. 102: 1557-1567, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DLK1

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Alternative Name: DLK1

Background:

Synonyms: Cyclin-dependent-like kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, CDK5, CDKN5

Tissue Specificity: Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary.

Background: Protein delta homolog 1 is a protein that in humans is encoded by the DLK1 gene. This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal.

Molecular Weight: 50 kDa

Gene ID: 8788

UniProt: P80370