NOS1AP antibody (AA 24-438)

Catalog No. ABIN7600833

This Rabbit Polyclonal antibody specifically detects NOS1AP in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NOS1AP antibody (AA 24-438) (ABIN7600833)

Target: NOS1AP (Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein (NOS1AP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1AP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NOS1AP Antibody

Binding Specificity: AA 24-438

Purpose: Anti-CAPON/NOS1AP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CAPON/NOS1AP Antibody Picoband® (ABIN7600833). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CAPON/NOS1AP recombinant protein (Position: D24-D438).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carrel, D., Du, Y., Komlos, D., Hadzimichalis, N. M., Kwon, M., Wang, B., Brzustowicz, L. M., Firestein, B. L. NOS1AP regulates dendrite patterning of hippocampal neurons through a carboxypeptidase E-mediated pathway. J. Neurosci. 29: 8248-8258, 2009. 2. Chang, K.-C., Barth, A. S., Sasano, T., Kizana, E., Kashiwakura, Y., Zhang, Y., Foster, D. B., Marban, E. CAPON modulates cardiac repolarization via neuronal nitric oxide synthase signaling in the heart. Proc. Nat. Acad. Sci. 105: 4477-4482, 2008. 3. Jaffrey, S. R., Snowman, A. M., Eliasson, M. J. L., Cohen, N. A., Snyder, S. H. CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95. Neuron 20: 115-124, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOS1AP

Target: NOS1AP (Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein (NOS1AP))

Alternative Name: NOS1AP

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4, USP15, KIAA0529

Tissue Specificity: Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.

Background: Nitric oxide synthase 1 adaptor protein (NOS1AP) also known as carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (CAPON) is a protein that in humans is encoded by the NOS1AP gene. This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins. Alternative splicing results in multiple transcript variants encoding different isoforms

Molecular Weight: 60 kDa

Gene ID: 9722

UniProt: O75052