DCT antibody (AA 24-519)

Catalog No. ABIN7600843

The Rabbit Polyclonal anti-DCT antibody has been validated for WB, ELISA and FACS. It is suitable to detect DCT in samples from Human, Mouse and Rat.

Quick Overview for DCT antibody (AA 24-519) (ABIN7600843)

Target: DCT (Dopachrome Tautomerase (DCT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-DCT Antibody

Binding Specificity: AA 24-519

Purpose: Anti-TRP2/DCT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRP2/DCT Antibody Picoband® (ABIN7600843). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRP2/DCT recombinant protein (Position: Q24-A519).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bouchard, B., Del Marmol, V., Jackson, I. J., Cherif, D., Dubertret, L. Molecular characterization of a human tyrosinase-related-protein-2 cDNA: patterns of expression in melanocytic cells. Europ. J. Biochem. 219: 127-134, 1994. 2. Budd, P. S., Jackson, I. J. Structure of the mouse tyrosinase-related protein-2/dopachrome tautomerase (Tyrp2/Dct) gene and sequence of two novel slaty alleles. Genomics 29: 35-43, 1995. 3. Cassady, J. L., Sturm, R. A. Sequence of the human dopachrome tautomerase-encoding TRP-2 cDNA. Gene 143: 295-298, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DCT

Target: DCT (Dopachrome Tautomerase (DCT))

Alternative Name: DCT

Background:

Synonyms: Beta-galactosidase, Glb1, Glb1_mapped, rCG_25043

Background: Dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2), also known as DCT, is a human gene. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteinerich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exist as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

Molecular Weight: 59 kDa

Gene ID: 1638

UniProt: P40126