SHARPIN antibody (AA 25-325)

Catalog No. ABIN7600904

This anti-SHARPIN antibody is a Rabbit Polyclonal antibody detecting SHARPIN in WB, ELISA, IHC and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for SHARPIN antibody (AA 25-325) (ABIN7600904)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHARPIN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SHARPIN Antibody

Binding Specificity: AA 25-325

Purpose: Anti-SHARPIN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SHARPIN Antibody (ABIN7600904). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHARPIN recombinant protein (Position: H25-R325). Human SHARPIN shares 72.3% and 72.6% amino acid (aa) sequence identity with mouse and rat SHARPIN, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Alternative Name: SHARPIN

Background:

Synonyms: SHARPIN, SIPL1, PSEC0216, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1

Background: Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium, protein linear polyubiquitination, and regulation of signal transduction. Located in cytosol. Part of LUBAC complex.

Molecular Weight: 40 kDa

Gene ID: 81858

UniProt: Q9H0F6