Methionine--tRNA Ligase, Cytoplasmic (AA 25-887) antibody

Catalog No. ABIN7600923

This Rabbit Polyclonal antibody specifically detects in ELISA, WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Methionine--tRNA Ligase, Cytoplasmic (AA 25-887) antibody (ABIN7600923)

Target: Methionine--tRNA Ligase, Cytoplasmic

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 25-887

Purpose: Anti-MARS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MARS1 Antibody Picoband® (ABIN7600923). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MARS1 recombinant protein (Position: R25-E887).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Botta, E., Theil, A. F., Raams, A., Caligiuri, G., Giachetti, S., Bione, S., Accadia, M., Lombardi, A., Smith, D. E. C., Mendes, M. I., Swagemakers, S. M. A., van der Spek, P. J., Salomons, G. S., Hoeijmakers, J. H. J., Yesodharan, D., Nampoothiri, S., Ogi, T., Lehmann, A. R., Orioli, D., Vermeulen, W. Protein instability associated with AARS1 and MARS1 mutations causes trichothiodystrophy. Hum. Molec. Genet. 30: 1711-1720, 2021. 2. Cirullo, R. E., Wasmuth, J. J. Assignment of the human gene encoding methionyl-tRNA synthetase to chromosome 12. (Abstract) Cytogenet. Cell Genet. 37: 437 only, 1984. 3. Cirullo, R. E., Wasmuth, J. J. Assignment of the human MARS gene, encoding methioninyl-tRNA synthetase, to chromosome 12 using human x Chinese hamster cell hybrids. Somat. Cell Molec. Genet. 10: 225-234, 1984.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Methionine--tRNA Ligase, Cytoplasmic

Alternative Name: MARS1

Background:

Synonyms: GTP-binding nuclear protein Ran,Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein,RAN,ARA24,OK/SW-cl.81,

Tissue Specificity: Expressed in a variety of tissues. .

Background: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.

Molecular Weight: 99 kDa

Gene ID: 4141

UniProt: P56192