BUB1 antibody (AA 26-1085)

Catalog No. ABIN7600942

The Rabbit Polyclonal anti-BUB1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect BUB1 in samples from Human, Mouse and Rat.

Quick Overview for BUB1 antibody (AA 26-1085) (ABIN7600942)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BUB1 Antibody

Binding Specificity: AA 26-1085

Purpose: Anti-BUB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BUB1 Antibody Picoband® (ABIN7600942). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BUB1 recombinant protein (Position: E26-K1085).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)" 2. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B (March 1998). "Mutations of mitotic checkpoint genes in human cancers". Nature 392 (6673): 300-3. 3. Roberts BT, Farr KA, Hoyt MA (December 1994). "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase". Mol. Cell. Biol. 14 (12): 8282-91.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1

Background:

Synonyms: RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS,

Tissue Specificity: Ubiquitous.

Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Molecular Weight: 130 kDa

Gene ID: 699

UniProt: O43683