IL4 Receptor antibody (AA 26-719)

Catalog No. ABIN7600970

This anti-IL4 Receptor antibody is a Rabbit Polyclonal antibody detecting IL4 Receptor in ELISA, IHC and FACS. Suitable for Mouse and Rat.

Quick Overview for IL4 Receptor antibody (AA 26-719) (ABIN7600970)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-IL4 Receptor Antibody

Binding Specificity: AA 26-719

Purpose: Anti-IL4R Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL4R Antibody Picoband® (ABIN7600970). Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL4R recombinant protein (Position: I26-E719).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL,
1. Bergin, A.-M., Balder, B., Kishore, S., Sward, K., Hahn-Zoric, M., Lowhagen, O., Hanson, L. A., Padyukov, L. Common variations in the IL4R gene affect splicing and influence natural expression of the soluble isoform. Hum. Mutat. 27: 990-998, 2006. 2. Blaeser, F., Bryce, P. J., Ho, N., Raman, V., Dedeoglu, F., Donaldson, D. D., Geha, R. S., Oettgen, H. C., Chatila, T. A. Targeted inactivation of the IL-4 receptor alpha chain I4R motif promotes allergic airway inflammation. J. Exp. Med. 198: 1189-1200, 2003. 3. Ikizawa, K, Yanagihara Y (Feb. 2000). "Possible involvement of Shc in IL-4-induced germline epsilon transcription in a human B cell line". Biochem. Biophys. Res.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: Il4r

Background:

Synonyms: Oncostatin-M, OSM, Osm

Tissue Specificity: Widely expressed. Expressed at higher levels in liver, skin and spleen.

Background: IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Molecular Weight: 180 kDa

Gene ID: 25084

UniProt: Q63257

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process