RDBP antibody (AA 262-378)

Catalog No. ABIN7600978

The Rabbit Polyclonal anti-RDBP antibody has been validated for WB, IHC, IF, ELISA, FACS and ICC. It is suitable to detect RDBP in samples from Human.

Quick Overview for RDBP antibody (AA 262-378) (ABIN7600978)

Target: RDBP (RD RNA Binding Protein (RDBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RDBP Antibody

Binding Specificity: AA 262-378

Purpose: Anti-NELFE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NELFE Antibody Picoband® (ABIN7600978). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NELFE recombinant protein (Position: N262-D378).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gibson, B. A., Zhang, Y., Jiang, H., Hussey, K. M., Shrimp, J. H., Lin, H., Schwede, F., Yu, Y., Kraus, W. L. Chemical genetic discovery of PARP targets reveals a role for PARP-1 in transcription elongation. Science 353: 45-50, 2016. 2. Levi-Strauss, M., Carroll, M. C., Steinmetz, M., Meo, T. A previously undetected MHC gene with an unusual periodic structure. Science 240: 201-204, 1988. 3. Narita, T., Yamaguchi, Y., Yano, K., Sugimoto, S., Chanarat, S., Wada, T., Kim, D., Hasegawa, J., Omori, M., Inukai, N., Endoh, M., Yamada, T., Handa, H. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex. Molec. Cell. Biol. 23: 1863-1873, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RDBP

Target: RDBP (RD RNA Binding Protein (RDBP))

Alternative Name: NELFE

Background:

Synonyms: Forkhead box protein F1, Forkhead-related activator 1, FREAC-1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FOXF1, FKHL5, FREAC1

Tissue Specificity: Expressed in kidney.

Background: The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins, however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

Molecular Weight: 43 kDa

Gene ID: 7936

UniProt: P18615