PGK2 antibody (AA 267-350)

Catalog No. ABIN7600983

This Rabbit Polyclonal antibody specifically detects PGK2 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PGK2 antibody (AA 267-350) (ABIN7600983)

Target: PGK2 (Phosphoglycerate Kinase 2 (PGK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PGK2 Antibody

Binding Specificity: AA 267-350

Purpose: Anti-PGK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PGK2 Antibody Picoband® (ABIN7600983). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PGK2 recombinant protein (Position: K267-K350).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, S.-H., Donahue, R. P., Scott, C. R. Characterization of phosphoglycerate kinase for human spermatozoa. Fertil. Steril. 27: 699-701, 1976. 2. Eicher, E. M., Cherry, M., Flaherty, L. Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex. Molec. Gen. Genet. 158: 225-228, 1978. 3. Gartler, S., Riley, D., Eddy, R., Shows, T. A human autosomal phosphoglycerate kinase gene has been assigned to chromosome 19. (Abstract) Cytogenet. Cell Genet. 40: 635-636, 1985.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PGK2

Target: PGK2 (Phosphoglycerate Kinase 2 (PGK2))

Alternative Name: PGK2

Background:

Synonyms: DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, Dj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, DNAJA2, CPR3, HIRIP4

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

Molecular Weight: 45 kDa

Gene ID: 5232

UniProt: P07205