PPP1R35 antibody (AA 27-253)

Catalog No. ABIN7601004

This Rabbit Polyclonal antibody specifically detects PPP1R35 in WB, ELISA, FACS, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for PPP1R35 antibody (AA 27-253) (ABIN7601004)

Target: PPP1R35 (Protein Phosphatase 1, Regulatory Subunit 35 (PPP1R35))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R35 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PPP1R35 Antibody

Binding Specificity: AA 27-253

Purpose: Anti-C7orf47/PPP1R35 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-C7orf47/PPP1R35 Antibody Picoband® (ABIN7601004). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human C7orf47/PPP1R35 recombinant protein (Position: E27-A253).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fong, C. S., Ozaki, K., Tsou, M.-F. B. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Molec. Biol. Cell 29: 2801-2808, 2018. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/1/2020. 3. Hendrickx, A., Beullens, M., Ceulemans, H., Abt, T. D., Van Eynde, A., Nicolaescu, E., Lesage, B., Bollen, M. Docking motif-guided mapping of the interactome of protein phosphatase-1. Chem. Biol. 16: 365-371, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPP1R35

Target: PPP1R35 (Protein Phosphatase 1, Regulatory Subunit 35 (PPP1R35))

Alternative Name: PPP1R35

Background:

Synonyms: Transmembrane protein 240,TMEM240,C1orf70,

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: PPP1R35 is a putative regulator of protein phosphatase-1. PPP1R35 is a centrosomal protein that regulates centriole elongation and centriole-to-centrosome conversion. Predicted to enable phosphatase binding activity and protein phosphatase inhibitor activity. Predicted to be involved in negative regulation of phosphatase activity.

Molecular Weight: 30 kDa

Gene ID: 221908