KLHL12 antibody (AA 27-331)

Catalog No. ABIN7601008

The Mouse Monoclonal anti-KLHL12 antibody has been validated for WB and IHC. It is suitable to detect KLHL12 in samples from Human, Mouse and Rat.

Quick Overview for KLHL12 antibody (AA 27-331) (ABIN7601008)

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLHL12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2G11D1

Product Details anti-KLHL12 Antibody

Binding Specificity: AA 27-331

Purpose: Anti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1) (ABIN7601008). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KLHL12 recombinant protein (Position: R27-I331).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/7/2012. 2. Jin, L., Pahuja, K. B., Wickliffe, K. E., Gorur, A., Baumgartel, C., Schekman, R., Rape, M. Ubiquitin-dependent regulation of COPII coat size and function. Nature 482: 495-500, 2012. 3. Mai, A., Jung, S.-K., Yonehara, S. hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure. Exp. Cell Res. 300: 72-83, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KLHL12

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Alternative Name: KLHL12

Background:

Synonyms: T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Kelch-like protein 12 is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Molecular Weight: 63 kDa

Gene ID: 59349

UniProt: Q53G59