ABHD4 antibody (AA 27-342)

Catalog No. ABIN7601011

The Rabbit Polyclonal anti-ABHD4 antibody has been validated for ELISA, WB and FACS. It is suitable to detect ABHD4 in samples from Human, Mouse and Rat.

Quick Overview for ABHD4 antibody (AA 27-342) (ABIN7601011)

Target: ABHD4 (Abhydrolase Domain Containing 4 (ABHD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABHD4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ABHD4 Antibody

Binding Specificity: AA 27-342

Purpose: Anti-ABHD4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ABHD4 Antibody Picoband® (ABIN7601011). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ABHD4 recombinant protein (Position: Q27-D342).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 1/27/2022. 2. Laszlo, Z. I., Lele, Z., Zoldi, M., Miczan, V., Mogor, F., Simon, G. M., Mackie, K., Kacskovics, I., Cravatt, B. F., Katona, I. ABHD4-dependent developmental anoikis safeguards the embryonic brain. Nature Commun. 11: 4363, 2020. 3. Lee, H.-C., Simon, G. M., Cravatt, B. F. ABHD4 regulates multiple classes of N-acyl phospholipids in the mammalian central nervous system. Biochemistry 54: 2539-2549, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ABHD4

Target: ABHD4 (Abhydrolase Domain Containing 4 (ABHD4))

Alternative Name: ABHD4

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: Predicted to enable lysophosphatidic acid acyltransferase activity and lysophospholipase activity. Predicted to be involved in N-acylphosphatidylethanolamine metabolic process, lipid homeostasis, and phosphatidic acid biosynthetic process. Predicted to act upstream of or within N-acylethanolamine metabolic process. Predicted to be located in endoplasmic reticulum membrane. Predicted to be active in lipid droplet and mitochondrion.

Molecular Weight: 39 kDa

Gene ID: 63874