CASK antibody (AA 27-498)

Catalog No. ABIN7601021

This anti-CASK antibody is a Rabbit Polyclonal antibody detecting CASK in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CASK antibody (AA 27-498) (ABIN7601021)

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CASK Antibody

Binding Specificity: AA 27-498

Purpose: Anti-CASK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CASK Antibody Picoband® (ABIN7601021). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CASK recombinant protein (Position: R27-D498).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Atasoy, D., Schoch, S., Ho, A., Nadasy, K. A., Liu, X., Zhang, W., Mukherjee, K., Nosyreva, E. D., Fernandez-Chacon, R., Missler, M., Kavalali, E. T., Sudhof, T. C. Deletion of CASK in mice is lethal and impairs synaptic function. Proc. Nat. Acad. Sci. 104: 2525-2530, 2007. 2. Butz, S., Okamoto, M., Sudhof, T. C. A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Cell 94: 773-782, 1998. 3. Cohen, A. R., Woods, D. F., Marfatia, S. M., Walther, Z., Chishti, A. H., Anderson, J. M. Human CASK/LIN-2 binds syndecan-2 and protein 4.1 and localizes to the basolateral membrane of epithelial cells. J. Cell Biol. 142: 129-138, 1998. Note: Erratum: J. Cell Biol. 142: following 1156, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CASK

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Alternative Name: CASK

Background:

Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Background: Peripheral plasma membrane protein CASK is a protein that in humans is encoded by the CASK gene. This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 120 kDa

Gene ID: 8573

UniProt: O14936

Pathways: Synaptic Vesicle Exocytosis