CES2 antibody (AA 27-549)

Catalog No. ABIN7601024

This anti-CES2 antibody is a Rabbit Polyclonal antibody detecting CES2 in WB, IHC, ELISA and IF. Suitable for Human and Mouse.

Quick Overview for CES2 antibody (AA 27-549) (ABIN7601024)

Target: CES2 (Carboxylesterase 2 (CES2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CES2 Antibody

Binding Specificity: AA 27-549

Purpose: Anti-CES2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CES2 Antibody Picoband® (ABIN7601024). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CES2 recombinant protein (Position: Q27-L549).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Pindel, E. V., Kedishvili, N. Y., Abraham, T. L., Brzezinski, M. R., Zhang, J., Dean, R. A., Bosron, W. F. Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin. J. Biol. Chem. 272: 14769-14775, 1997. 2. Sanghani, S. P., Quinney, S. K., Fredenburg, T. B., Davis, W. I., Murry, D. J., Bosron, W. F. Hydrolysis of irinotecan and its oxidative metabolites, 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino] carbonyloxycamptothecin and 7-ethyl-10-[4-(1-piperidino)-1-amino]-carbonyloxycamptothecin by human carboxylesterases CES1A1, CES2, and a newly expressed carboxylesterase isoenzyme, CES3. Drug Metab. Dispos. 32: 505-513, 2004. 3. Schwer, H., Langmann, T., Daig, R., Becker, A., Aslanidis, C., Schmitz, G. Molecular cloning and characterization of a novel putative carboxylesterase, present in human intestine and liver. Biochem. Biophys. Res. Commun. 233: 117-120, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CES2

Target: CES2 (Carboxylesterase 2 (CES2))

Alternative Name: CES2

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 13C, B-cell maturation defect, B-cell-activating factor receptor, BAFF receptor, BAFF-R, BLyS receptor 3, CD268, Tnfrsf13c, Baffr, Bcmd, Br3

Tissue Specificity: Highly expressed in spleen and testis, detected at lower levels in lung and thymus.

Background: Carboxylesterase 2 is an enzyme that in humans is encoded by the CES2 gene. This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 62 kDa

Gene ID: 8824

UniProt: O00748