DNMT3B antibody (AA 271-853)

Catalog No. ABIN7601041

The Rabbit Polyclonal anti-DNMT3B antibody has been validated for WB, ELISA and FACS. It is suitable to detect DNMT3B in samples from Human, Mouse, Rat and Monkey.

Quick Overview for DNMT3B antibody (AA 271-853) (ABIN7601041)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-DNMT3B Antibody

Binding Specificity: AA 271-853

Purpose: Anti-DNMT3B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DNMT3B Antibody Picoband® (ABIN7601041). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DNMT3B recombinant protein (Position: E271-E853).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Balada, E., Ordi-Ros, J., Serrano-Acedo, S., Martinez-Lostao, L., Rosa-Leyva, M., Vilardell-Tarres, M. Transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus. Immunology 124: 339-347, 2008. 2. Barau, J., Teissandier, A., Zamudio, N., Roy, S., Nalesso, V., Herault, Y., Guillou, F., Bourc'his, D. The DNA methyltransferase DNMT3C protects male germ cells from transposon activity. Science 354: 909-912, 2016. 3. Beaulieu, N., Morin, S., Chute, I. C., Robert, M.-F., Nguyen, H., MacLeod, A. R. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J. Biol. Chem. 277: 28176-28181, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: DNMT3B

Background:

Synonyms: Copper chaperone for superoxide dismutase,Superoxide dismutase copper chaperone,CCS,

Tissue Specificity: Ubiquitous.

Background: DNA (cytosine-5-)-methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Molecular Weight: 100 kDa

Gene ID: 1789

UniProt: P50570