PDLIM7 antibody (AA 275-457)

Catalog No. ABIN7601048

This anti-PDLIM7 antibody is a Rabbit Polyclonal antibody detecting PDLIM7 in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PDLIM7 antibody (AA 275-457) (ABIN7601048)

Target: PDLIM7 (PDZ and LIM Domain 7 (Enigma) (PDLIM7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PDLIM7 Antibody

Binding Specificity: AA 275-457

Purpose: Anti-PDLIM7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDLIM7 Antibody Picoband® (ABIN7601048). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDLIM7 recombinant protein (Position: N275-V457).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bach, I. The LIM domain: regulation by association. Mech. Dev. 91: 5-17, 2000. 2. Durick, K., Gill, G. N., Taylor, S. S. Shc and Enigma are both required for mitogenic signaling by Ret/ptc2. Molec. Cell. Biol. 18: 2298-2308, 1998. 3. Guy, P. M., Kenny, D. A., Gill, G. N. The PDZ domain of the LIM protein enigma binds to beta-tropomyosin. Molec. Biol. Cell 10: 1973-1984, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDLIM7

Target: PDLIM7 (PDZ and LIM Domain 7 (Enigma) (PDLIM7))

Alternative Name: PDLIM7

Background:

Synonyms: "Long-chain-fatty-acid--CoA ligase 4, Long-chain acyl-CoA synthetase 4, LACS 4, ACSL4, ACS4, FACL4, LACS4 "

Tissue Specificity: Expressed in the prostate gland and prostate cancers.

Background: PDZ and LIM domain protein 7 is a protein that in humans is encoded by the PDLIM7 gene. The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 50 kDa

Gene ID: 9260