TOE1 antibody (AA 28-501)

Catalog No. ABIN7601080

The Rabbit Polyclonal anti-TOE1 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect TOE1 in samples from Human.

Quick Overview for TOE1 antibody (AA 28-501) (ABIN7601080)

Target: TOE1 (Target of EGR1, Member 1 (Nuclear) (TOE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TOE1 Antibody

Binding Specificity: AA 28-501

Purpose: Anti-TOE1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TOE1 Antibody Picoband® (ABIN7601080). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TOE1 recombinant protein (Position: E28-Q501).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anderson, C., Davies, J. H., Lamont, L., Foulds, N. Early pontocerebellar hypoplasia with vanishing testes: a new syndrome? Am. J. Med. Genet. 155A: 667-672, 2011. 2. Brault, L. S., Cooper, C. A., Famula, T. R., Murray, J. D., Penedo, M. C. T. Mapping of equine cerebellar abiotrophy to ECA2 and identification of a potential causative mutation affecting expression of MUTYH. Genomics 97: 121-129, 2011. 3. de Belle, I., Wu, J.-X., Sperandio, S., Mercola, D., Adamson, E. D. In vivo cloning and characterization of a new growth suppressor protein TOE1 as a target gene of Egr1. J. Biol. Chem. 278: 14306-14312, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TOE1

Target: TOE1 (Target of EGR1, Member 1 (Nuclear) (TOE1))

Alternative Name: TOE1

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: Target of EGR1 protein 1 is a protein that in humans is encoded by the TOE1 gene. Enables poly(A)-specific ribonuclease activity and snRNA binding activity. Involved in RNA phosphodiester bond hydrolysis, exonucleolytic and snRNA 3'-end processing. Located in Cajal body and cytoplasm. Implicated in pontocerebellar hypoplasia type 7.

Molecular Weight: 57 kDa

Gene ID: 114034