GPC5 antibody (AA 28-538)

Catalog No. ABIN7601084

This anti-GPC5 antibody is a Rabbit Polyclonal antibody detecting GPC5 in WB, ELISA, IF, ICC and FACS. Suitable for Human.

Quick Overview for GPC5 antibody (AA 28-538) (ABIN7601084)

Target: GPC5 (Glypican 5 (GPC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GPC5 Antibody

Binding Specificity: AA 28-538

Purpose: Anti-Glypican 5/GPC5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Glypican 5/GPC5 Antibody Picoband® (ABIN7601084). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Glypican 5/GPC5 recombinant protein (Position: Q28-D538).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Neat, M. J., Foot, N., Jenner, M., Goff, L., Ashcroft, K., Burford, D., Dunham, A., Norton, A., Lister, T. A., Fitzgibbon, J. Localisation of a novel region of recurrent amplification in follicular lymphoma to an ~6.8 Mb region of 13q32-33. Genes Chromosomes Cancer 32: 236-243, 2001. 2. Veugelers, M., Vermeesch, J., Reekmans, G., Steinfeld, R., Marynen, P., David, G. Characterization of glypican-5 and chromosomal localization of human GPC5, a new member of the glypican gene family. Genomics 40: 24-30, 1997. 3. Yu, W., Inoue, J., Imoto, I., Matsuo, Y., Karpas, A., Inazawa, J. GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J. Hum. Genet. 48: 331-335, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GPC5

Target: GPC5 (Glypican 5 (GPC5))

Alternative Name: GPC5

Background:

Synonyms: Formin-1,Limb deformity protein homolog,FMN1,FMN, LD,

Tissue Specificity: Expressed ubiquitously.

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Molecular Weight: 64 kDa

Gene ID: 2262

UniProt: P78333

Pathways: Glycosaminoglycan Metabolic Process