GSTA4 antibody (AA 29-222)

Catalog No. ABIN7601113

The Rabbit Polyclonal anti-GSTA4 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect GSTA4 in samples from Human and Monkey.

Quick Overview for GSTA4 antibody (AA 29-222) (ABIN7601113)

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-GSTA4 Antibody

Binding Specificity: AA 29-222

Purpose: Anti-GSTA4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GSTA4 Antibody Picoband® (ABIN7601113). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GSTA4 recombinant protein (Position: E29-P222).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Atkinson, HJ, Babbitt, PC (Nov 24, 2009). "Glutathione transferases are structural and functional outliers in the thioredoxin fold.". Biochemistry 48 (46): 11108-16. 2. Boyer TD (March 1989). "The glutathione S-transferases: an update". Hepatology 9(3): 486-96.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GSTA4

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Alternative Name: GSTA4

Background:

Synonyms: Tissue factor pathway inhibitor 2,TFPI-2,Placental protein 5,PP5,TFPI2,

Tissue Specificity: Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

Background: Glutathione S-transferase A4, also known as GSTA4, is an enzyme which in humans is encoded by the GSTA4 gene. Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules). In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity, thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Molecular Weight: 26 kDa

Gene ID: 221357

UniProt: O15217