C5AR1 antibody (AA 29-327)

Catalog No. ABIN7601126

This anti-C5AR1 antibody is a Rabbit Polyclonal antibody detecting C5AR1 in WB, FACS, ELISA, IF and ICC. Suitable for Mouse.

Quick Overview for C5AR1 antibody (AA 29-327) (ABIN7601126)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C5AR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-C5AR1 Antibody

Binding Specificity: AA 29-327

Purpose: Anti-C5a-R/C5ar1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-C5a-R/C5ar1 Antibody Picoband® (ABIN7601126). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse C5a-R/C5ar1 recombinant protein (Position: H29-D327).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Arbore, G., West, E. E., Spolski, R., Robertson, A. A. B., Klos, A., Rheinheimer, C., Dutow, P., Woodruff, T. M., Yu, Z. X., O'Neill, L. A., Coll, R. C., Sher, A., and 10 others. T helper 1 immunity requires complement-driven NLRP3 inflammasome activity in CD4+ T cells. Science 352: aad1210, 2016 2. Atkinson, J. P. Complement system on the attack in autoimmunity. (Commentary) J. Clin. Invest. 112: 1639-1641, 2003. 3. Bao, L., Gerard, N. P., Eddy, R. L., Jr., Shows, T. B., Gerard, C. Mapping of genes for the human C5a receptor (C5AR), human FMLP receptor (FPR), and two FMLP receptor homologue orphan receptors (FPRH1, FPRH2) to chromosome 19. Genomics 13: 437-440, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5ar1

Background:

Synonyms: C-C chemokine receptor type 1,C-C CKR-1,CC-CKR-1,CCR-1,CCR1,Macrophage inflammatory protein 1-alpha receptor,MIP-1alpha-R,RANTES-R,CD191,Ccr1,Cmkbr1,

Tissue Specificity: Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

Background: The C5a receptor also known as complement component 5a receptor 1 (C5AR1) or CD88 (Cluster of Differentiation 88) is a G protein-coupled receptor for C5a. It functions as a complement receptor. C5a receptor modulates inflammatory responses, obesity, development and cancers.

Molecular Weight: 43 kDa

Gene ID: 12273

UniProt: P30993

Pathways: Complement System