CD276 antibody (AA 29-458)

Catalog No. ABIN7601136

This Rabbit Polyclonal antibody specifically detects CD276 in WB, ELISA, FACS and IHC. It exhibits reactivity toward Human.

Quick Overview for CD276 antibody (AA 29-458) (ABIN7601136)

Target: CD276

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD276 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-CD276 Antibody

Binding Specificity: AA 29-458

Purpose: Anti-CD276 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti- Antibody Picoband® (ABIN7601136). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD276 recombinant protein (Position: L29-Q458).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chapoval, A. I., Ni, J., Lau, J. S., Wilcox, R. A., Flies, D. B., Liu, D., Dong, H., Sica, G. L., Zhu, G., Tamada, K., Chen, L. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nature Immun. 2: 269-274, 2001. 2. Steinberger, P., Majdic, O., Derdak, S. V., Pfistershammer, K., Kirchberger, S., Klauser, C., Zlabinger, G., Pickl, W. F., Stockl, J., Knapp, W. Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains. J. Immun. 172: 2352-2359, 2004. 3. Suh, W.-K., Gajewska, B. U., Okada, H., Gronski, M. A., Bertram, E. M., Dawicki, W., Duncan, G. S., Bukczynski, J., Plyte, S., Elia, A., Wakeham, A., Itie, A., and 12 others. The B7 family member B7-H3 preferentially down-regulates T helper type 1-mediated immune responses.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD276

Target: CD276

Alternative Name: CD276

Background:

Synonyms: Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, CD131, Csf2rb, Aic2b, Csf2rb1, Il3rb1

Tissue Specificity: Broadly expressed with higher expression in heart.

Background: Cluster of Differentiation 276 (CD276) or B7 Homolog 3 (B7-H3) is a human protein encoded by the CD276 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 90-100 kDa

Gene ID: 80381

Pathways: Cancer Immune Checkpoints