TRMT5 antibody (AA 291-501)

Catalog No. ABIN7601151

Product Details anti-TRMT5 Antibody

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Binding Specificity: AA 291-501

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Anti-TRMT5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRMT5 Antibody Picoband® (ABIN7601151). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRMT5 recombinant protein (Position: R291-E501).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Argente-Escrig, H., Vilchez, J. J., Frasquet, M., Muelas, N., Azorin, I., Vilchez, R., Millet-Sancho, E., Pitarch, I., Tomas-Vila, M., Vazquez-Costa, J. F., Mas-Estelles, F., Marco-Marin, C., Espinos, C., Serrano-Lorenzo, P., Martin, M. A., Lupo, V., Sevilla, T. A novel TRMT5 mutation causes a complex inherited neuropathy syndrome: The role of nerve pathology in defining a demyelinating neuropathy. Neuropath. Appl. Neurobiol. 48: e12817, 2022. 2. Brule, H., Elliott, M., Redlak, M., Zehner, Z. E., Holmes, W. M. Isolation and characterization of the human tRNA-(N(1)G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein. Biochemistry 43: 9243-9255, 2004. 3. Haller, R. G., Lewis, S. F., Estabrook, R. W., DiMauro, S., Servidei, S., Foster, D. W. Exercise intolerance, lactic acidosis, and abnormal cardiopulmonary regulation in exercise associated with adult skeletal muscle cytochrome c oxidase deficiency. J. Clin. Invest. 84: 155-161, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRMT5

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Alternative Name: TRMT5 (TRMT5 Products)

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.

Molecular Weight: 50 kDa

Gene ID: 57570

UniProt: Q32P41