TRMT5 antibody (Middle Region)

Catalog No. ABIN2783538

The Rabbit Polyclonal anti-TRMT5 antibody is suitable to detect TRMT5 in samples from Human, Rat, Horse, Rabbit, Cow, Dog and Pig. It has been validated for WB.

Quick Overview for TRMT5 antibody (Middle Region) (ABIN2783538)

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Reactivity: Human, Rat, Horse, Rabbit, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRMT5 Antibody

Binding Specificity: Middle Region

Purpose: TRMT5 Antibody - middle region

Sequence: EMLCITFQIP ASVLYKNQTR NPENHEDPPL KRQRTAEAFS DEKTQIVSNT

Predicted Reactivity: Cow: 77%, Dog: 85%, Horse: 83%, Human: 100%, Pig: 77%, Rabbit: 75%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against TRMT5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRMT5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48868-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRMT5

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Alternative Name: TRMT5

Background:

Background Information: tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM].

Gene Name: TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)

Alternative Symbols: TRM5, COXPD26, KIAA1393

Protein Name: tRNA (guanine(37)-N1)-methyltransferase

Molecular Weight: 58kDa

Gene ID: 57570

NCBI Accession: NP_065861

UniProt: Q32P41