RAD21 antibody (AA 292-529)

Catalog No. ABIN7601152

The Rabbit Polyclonal anti-RAD21 antibody has been validated for WB, ELISA, IF, FACS and ICC. It is suitable to detect RAD21 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for RAD21 antibody (AA 292-529) (ABIN7601152)

Target: RAD21 (RAD21 Homolog (RAD21))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RAD21 Antibody

Binding Specificity: AA 292-529

Purpose: Anti-RAD21 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RAD21 Antibody (ABIN7601152). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAD21 recombinant protein (Position: D292-K529).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: RAD21

Background:

Synonyms: Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, OK/SW-cl.56

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Double-strand-break repair protein rad21 homolog is a protein that in humans is encoded by the RAD21 gene. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Molecular Weight: 72 kDa

Gene ID: 5885

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity