AKR7A2 antibody (AA 294-359)

Catalog No. ABIN7601157

This Rabbit Polyclonal antibody specifically detects AKR7A2 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AKR7A2 antibody (AA 294-359) (ABIN7601157)

Target: AKR7A2 (Aflatoxin Beta1 Aldehyde Reductase (AKR7A2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR7A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-AKR7A2 Antibody

Binding Specificity: AA 294-359

Purpose: Anti-AKR7A2 Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AKR7A2 Picoband® Antibody (ABIN7601157). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AKR7A2 recombinant protein (Position: A294-R359).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ireland, L. S., Harrison, D. J., Neal, G. E., Hayes, J. D. Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B(1)-aldehyde reductase. Biochem. J. 332: 21-34, 1998. 2. Kelly, V. P., Sherratt, P. J., Crouch, D. H., Hayes, J. D. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of reductase 7 family proteins. Biochem. J. 366: 847-861, 2002. 3. Praml, C., Savelyeva, L., Schwab, M. Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells. Oncogene 22: 4765-4773, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AKR7A2

Target: AKR7A2 (Aflatoxin Beta1 Aldehyde Reductase (AKR7A2))

Alternative Name: AKR7A2

Background:

Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7

Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

Background: Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It is mapped to 1p36.13. The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36 kDa

Gene ID: 8574

UniProt: O43488