LIG3 antibody (AA 297-963)

Catalog No. ABIN7601167

The Rabbit Polyclonal anti-LIG3 antibody has been validated for WB, ELISA and FACS. It is suitable to detect LIG3 in samples from Human, Mouse and Rat.

Quick Overview for LIG3 antibody (AA 297-963) (ABIN7601167)

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LIG3 Antibody

Binding Specificity: AA 297-963

Purpose: Anti-DNA Ligase III/LIG3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DNA Ligase III/LIG3 Antibody Picoband® (ABIN7601167). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DNA Ligase III/LIG3 recombinant protein (Position: D297-D963).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, J., Tomkinson, A. E., Ramos, W., Mackey, Z. B., Danehower, S., Walter, C. A., Schultz, R. A., Besterman, J. M., Husain, I. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Molec. Cell. Biol. 15: 5412-5422, 1995. 2. Gao, Y., Katyal, S., Lee, Y., Zhao, J., Rehg, J. E., Russell, H. R., McKinnon, P. J. DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair. Nature 471: 240-244, 2011. 3. Moser, J., Kool, H., Giakzidis, I., Caldecott, K., Mullenders, L. H. F., Fousteri, M. I. Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III-alpha in a cell-cycle-specific manner. Molec. Cell 27: 311-323, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: LIG3

Background:

Synonyms: Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, Small-inducible cytokine B7, PPBP, CTAP3, CXCL7, SCYB7, TGB1, THBGB1, NAP-2

Background: DNA ligase 3 is an enzyme that, in humans, is encoded by the LIG3 gene. This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 113 kDa

Gene ID: 3980

UniProt: P49916

Pathways: DNA Damage Repair