RPL10 antibody (AA 3-214)

Catalog No. ABIN7601177

This anti-RPL10 antibody is a Rabbit Polyclonal antibody detecting RPL10 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for RPL10 antibody (AA 3-214) (ABIN7601177)

Target: RPL10 (Ribosomal Protein L10 (RPL10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-RPL10 Antibody

Binding Specificity: AA 3-214

Purpose: Anti-RPL10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RPL10 Antibody Picoband® (ABIN7601177). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPL10 recombinant protein (Position: R3-S214).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brooks, S. S., Wall, A. L., Golzio, C., Reid, D. W., Kondyles, A., Willer, J. R., Botti, C., Nicchitta, C. V., Katsanis, N., Davis, E. E. A novel ribosomopathy caused by dysfunction of RPL10 disrupts neurodevelopment and causes X-linked microcephaly in humans. Genetics 198: 723-733, 2014. 2. Chiocchetti, A., Pakalapati, G., Duketis, E., Wiemann, S., Poustka, A., Poustka, F., Klauck, S. M. Mutation and expression analyses of the ribosomal protein gene RPL10 is an extended German sample of patients with autism spectrum disorder. Am. J. Med. Genet. 155A: 1472-1475, 2011. 3. De Keersmaecker, K., Atak, Z. K., Li, N., Vicente, C., Patchett, S., Girardi, T., Gianfelici, V., Geerdens, E., Clappier, E., Porcu, M., Lahortiga, I., Luca, R., and 18 others. Exome sequencing identifies mutation in CNOT3 and ribosomal genes RPL5 and RPL10 in T-cell acute lymphoblastic leukemia. Nature Genet. 45: 186-190, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RPL10

Target: RPL10 (Ribosomal Protein L10 (RPL10))

Alternative Name: RPL10

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, Mtnd4, mt-Nd4, Nd4

Tissue Specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

Background: 60S ribosomal protein L10 is a protein that in humans is encoded by the RPL10 gene. This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. Some studies have detected an association between variation in this gene and autism spectrum disorders, though others do not detect this relationship. There are multiple pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants.

Molecular Weight: 23 kDa

Gene ID: 6134

UniProt: P27635