ASS1 antibody (AA 3-365)

Catalog No. ABIN7601191

The Mouse Monoclonal anti-ASS1 antibody is suitable to detect ASS1 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for ASS1 antibody (AA 3-365) (ABIN7601191)

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 5I5

Product Details anti-ASS1 Antibody

Binding Specificity: AA 3-365

Purpose: Anti-ASS1 Antibody (monoclonal, 5I5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ASS1 Antibody (monoclonal, 5I5) (ABIN7601191). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ASS1 recombinant protein (Position: S3-S365).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Alternative Name: ASS1

Background:

Synonyms: Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. It is mapped to 9q34.11. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 47 kDa

Gene ID: 445

UniProt: P00966

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin